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Background&Objective: Considering the high prevalence of Tricomonas vaginalis (TV) in women and the known side effects of metronidazol, herbal drug therapy in order to reduce drug side effects has been considered increasingly in recent decades. This study was done to determine the effect of Artemisia aucheri Boiss, Zataria multiflora Boiss and Myrtus communis L. on Trichomonas vaginalis. Materials&Methods: This study was done on samples extracted from 100 patients with vaginitis due to Trichomona. The parasite was isolated from vagina and determined directly. Identification was done through direct smear preparation. Parasite was added to the 5 test tubes containing Dorse medium, metronidazole. dimethyl sulfoxaide (DMSO), Artemisia, Zataria and Myrtus extraction with concentration of 0.1, 0.01 ml in order to determine the effect of these concentrations within 72 hour. Results: Trichomonas could be alived in Dorse medium for 72 hours, in presence of metronidazole for one hour and in Dorse medium for 6 hours. Also, methonolic extracts of Artemisia are effective at concentration of 0.1 after one hour and 0,01 after 4 hours of the inoculation. Methanolic extracts of Zataria at concentrations of 0.1 and 0.01 and the Myrtus extract at concentration of 0.1 are effective at beginning of inoculation but the Myrtus extract at concentration of 0.01 is effective after one hour. Conclusion: Considering the acceptable effect of metanolic extracts of these plants on trichomonas in in-vitro conditions, it is recommended that , the therapeutic effects of the substances from these plants to be studied in in vivo conditions and in case of having positive effect to be used as a drug.

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Background & Objective: There is an increasing interests in substitution of natural products for synthetic chemicals in drugs and foodstuff. Reports of antibiotic resistant bacteria and side effects of chemical food preservatives suspected to be carcinogenic have drawn attention towards application of herbal and natural substances. Towards this end, edible plants are more safe and therefor have priority for investigation. In this study, antibacterial activities of six medicinal plants including Thymus (T.) eriocalyx and T. persicus, Allium (A.) haementhaides, Fumaria (F.) parviflora Lam, Buxus (B.) hyrecana pojark, and Tragopon (Tr.) carcifolus Lorestan province in Iran were examined. Materials & Methods: In this study, flowers and leaves from T.persicus, T. eriocalyx, and leaves from other plants were collected from Zagros highlands, dried and soaked in hexan for 48. The solvent was separated then evaporated under reduced pressure. The concentrated extracts were shaken and foze at -10°C. Then it was centrifuged and the solvent was evaporated. The extracts were finally resuspended in sterile PBS containing DMSO. Antibacterial activities were examined by disk diffusion and broth microdillution technique , using standard (ATCC) gram positive and negative bacteria and standard (Muller Hinton agar and broth or Isosensitest agar) media in order to determine Minimum Inhibitory (MIC) or Bactericidal (MBC) concentration. Results: Strong antibacterial activities were observed against both gram positive and negative bacteria including E.coli, S. aureous and P. aeroginosa by T.eriocalyx and F. parviflora. Also B. hyrecana pojark showed antibacterial activity against S. aureus at a concentration of MIC=320 µg/ml and MBC=80 µg/ml. Conclusion: This study showed that T.eriocalyx, F. parviflora and B. hyrecana pojark have antibacterial effects in vitro. Therefore it is suggested the application of these extract in medicine and food industings could be helpful.