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Background & Objective: Adenosine deaminase is an enzyme which catalyses adenosine to Inosine. The determination of adenosine deaminase in body fluids is important for the diagnosis of tuberculosis. There are contradictory reports about the diagnostic value of serum adenosine deaminase in pulmonary tuberculosis. This study was set up to investigate the diagnostic value of serum adenosine deaminase and its isoenzymes activities on pulmonary tuberculosis. Materials & Methods: In this descriptive study, blood samples were obtained from 26pulmonary tuberculosis patients (group 1), 17 suspected tuberculosis with negative in both smear and culture tests (group 2), and 67 healthy subjects (group 3). Total ADA and ADA2 determination was carried out by kinetic method and EHNA Inhibitor, respectively. Results: ADA and ADA2 activities are as follow: 19.35±5.04, 13.35±5.34 (group 1) 17.24±6.20, 11.47±3.92 (group 2) and 13.96±4.25, 7.36±2.91(group 3). The mean differences of ADA and ADA2 activity between group 1 and 2 with group 3 was meaningful. The sensitivity and specificity for ADA and ADA2 tests were (26.9%, 94 %) and (50%, 97 %) respectively. The PPV for ADA and ADA2 were 63.6% and 86.7% and the NPV were 76.8% and 83.3%, respectively. Conclusion: This study indicated that the assessment of these enzymes in serum to some extend can be a useful method for differentiation of healthy subjects from respiratory disease, but these tests do not have enough sensitivity to assist in the diagnoses of tuberculosis patients from other respiratory diseases.

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Background and Objective: Stroke and cerebral ischemia are the second leading causes of death in the world. Currently, there are limited therapeutic interventions for patients with ischemia / reperfusion. This study was performed to determine the protective effect of aerobic exercise and adenosine on changes in inflammation mediators after transient ischemia of common carotid arteries in male Wistar rats.
Methods: In this experimental study, fifty male Wistar rats were randomly allocated into to 5 groups: control, cerebral ischemic control, aerobic exercise + cerebral ischemia, adenosine + cerebral ischemia and aerobic exercise + adenosine + cerebral ischemia. Ischemia was performed by blocking the common carotid artery for 45 minutes after a period of exercise and injection of adenosine. Neuronal structure was examined by Nissel tissue staining. The expression of NGF and Glutamate genes were measured in CA1 region of hippocampal tissue samples.
Results: Cell death was increased in neurons in the CA1 region of the hippocampus in the ischemia / reperfusion group, While a significant reduction in cell death in the adenosine + ischemia / reperfusion and aerobic exercise + ischemia /reperfusion groups was due to adenosine administration and aerobic exercise (P<0.05). NGF and glutamate gene expression in the adenosine + ischemia/reperfusion and adenosine + aerobic exersice + ischemia/reperfusion groups significantly increased and reduced compared to the ischemia/reperfusion control, respectively (P<0.05).
Conclusion: Co-administration of adenosine combined with aerobic exercise increase the protective effect of aerobic exercise on improving the neuronal damage after ischemic / reperfusion.