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Background&Objective: The Failure progress of labor is the second indication for cesarean delivery after repeat section. It is generally agreed that dystocia leading ro cesarean delivery is overdiagnosed in the world. Variability in the criteria for diagnosis is major determinant of the increase in cesarean deliveries for dystocia. This study was identified dystocia with comparison with the criteria obstetrics standards and determined the cause's effects of incorrect diagnosis. Materials&Methods: A descriptive analytic case-control study was performed on 1212 women who experienced cesarean delivery in Yahyanegad hospital of Babol a city in North of Iran during 2004. We compared criteria of diagnosis of dystocia among 248 women whome were operated with dystocia with criteria that were proposed by American college of obstetricians and gynecologis. Also, we compared 118 women with correct diagnosis with 130 women with incorrect diagnosis to identify causes of incorrect diagnosis. Statistical test 2, t-Test and multiple logistic regressions were used to analysis of data. Results: Dystocia was diagnosed during latent phase, active phase and second stage respectively: %64.9, %29.8 and %5.3. The strogenst predictor of incorrect diagnosis of dystocia was the lack of administration of oxytocine. Other independent risk factors for failure of labor to progress, using a multivariable analysis, were: performing of cesarean in morning (OR=2.8 %95 CI 2.1 –3.5), performing of cesarean in afternoon (OR = 2.6 %95 CI 1.3-3), nulliparity (OR=2.1 %95 CI 1.7-3.2), incorrect clinical estimation of fetal macrosomia (OR= 2.3 %95 CI 1.5-2.9). Conclusion: %50 accuracy of failure of labor progress according to obstetrics standards implies that there is overdiagnosis in the dystocia. This study proposes that obstetricans should be managed conservatively protract dilatation in the patients to prevent of incorrect diagnosed dystocia cesarean. Active management of labor with accurate administration of oxytocine and amniotomy is another strategy that may be help to decrease cesarean with diagnostic dystocia.

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Background&Objective: Low back pain (LBP) is common in youth, although `associated with chronic pain in adults. The purpose of this study was to determine the prevalence and risk factors of LBP in school children age 11-14. Materials&Methods: A cross sectional study was carried out on school children aged 11-14 in north of Iran. Using random multistage sampling 5000 children were selected. Demographic, mechanical and lifestyle data as well as the prevalence and LBP characteristics were collected by a questionnaire. Data analysis was carried out using SPSS (version11) software. Results: Of the original sample, 4813 (96.2%) children returned the questionnaire. Point, last month, last 6 months and annual prevalence were 15%, 14.4%, 15.6%, and 17.4%, respectively. LBP was significantly correlated with age (p<0.05), lifting (p<0.05), position and duration of watching TV, doing homework and duration of exercise (p<0.05). Conclusion: The results indicated that the prevalence of LBP in school children is relatively high. Further studies evaluating the effect of different preventive strategies to reduce the prevalence of LBP in school children is strongly recommended.

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Background&Objective: Consultation prior to blood donation and screening tests of common blood born diseases such as Hepatitis B,C and AIDS are very important issue in blood quality assurance, therefore we decided to compare frequency of such diseases in blood donors and outpatients admitted in the blood transfusion centers. Materials&Methods: For this purpose the initial tests including HBsAg, HCVAb, and HIVAb were performed on all blood donors and outpatients' blood samples in Gorgan transfusion center by ELIZA methods during 2003 and confirmatory tests including neutralization for HBsAg, RIBA for HCVAb and Western blot for HIVAb performed in condition where ELIZA report was positive. Results: The blood donor in this study had the following positive results: HbsAg 2.5%, HCVAb 3%, HIV 0.35%. The same indeces for the out patients were positive as follow: 13%, 8.3% and 2.04% respectively. The other finding from this investigation indicate that the blood donors positive cases with the ELISA technique were confirmed by Western blot 92%, 20.1% nad 0% for HBSAg, HCVAb, as HIV respectively. The confimatory results of the positive out patient's cases were as follow, 97%, 47% and 60% for HBSAg, HCVAb and HIV respectivley. Conclusion: This study reveals frequency of Hepatitis B and C and AIDS among outpatients which considered being high risk group are more than the blood donors. We conclude that the measurement of HBSAg by ELIAZ methodes in comparision to the HCV and HIV are more accurate.

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Background&Objective: Burning and burn wound are the causes of mortality in the world. The present study was designed to investigate the effect of egg yolk (of hen) on burn wound healing in Rats. Materials&Methods: In this experimental sutdy, 48 females rats randomely divided in silver sulphadiazin, egg yolk and control groups, respectively. Each of three groups including 16 rats and was selected for 28 days of treatment. After general anesthesia with ketamin and xylazin (ip) an area of the back of rats was burned with red-hot coin and similar grade II burns were created. Control group received no medication. In the second and third groups, the surface of wound was covered daily with sulphadiazine and egg yolk. At the end of the eack week, four rats from the each group were scarified by ether and samples were taken from the wound region and were histologically assessed. The samples were stanied with Hematoxillin and Eosin and studied quantitively. Results: Results showed that after 4 weeks the healing of burn wounds in the groups receiving egg yolk and sulphadiazine were similar. But healing of burn wounds within these 2 groups was better than control group. There was no difference between the groups receiving either egg yolk or sulphadiazin. Conclusion: This study showed that egg yolk can be used as an alternative to sulphadiazin in second degree burn wounds.

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Background&Objective: The evaluation and measurement of human body dimensions are achieved by physical anthropometry. Cephalomerty is a branch of antrompometry Science, in which the head and face anatomical are measured. With respect to its importance in legal medicine, plastic surgery, radiology, anatomy, orthodency and industry, this study was set up to determine and compare the face shapes in Fars and Turkman ethnic groups of normal female adults. Materials&Methods: This study was a descriptive-and cross-sectional study which was carried out on 410 girls of 17-20 years of age in two ethnic groups of native Fars and Turkman. The length and width of girls face were determined by using classic cephalometry techniqre. On the basis of this method, the shape of faces was subdivided in five interrational groups. Subsequently the prevalancy and the shape of faces in the ethnic group of Fars and Turkman were compared. Results: The prosopic (facial) indices of Fars and Turkman groups were 84.55.8 and 81.55.2, respectively. Dominant shape of face in Fars group was euryprosopic (37.7%) and in Turkman group was euryprosopic (51.7%). Rare shape of face in Fars and Turkman groups were hyperleptoprosopic (5.8%) and leptoprosopic (3%) respectively. Conclusion: This study showed that the face shape is affeiced by ethnical factor and primarily by genetic factor.

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Background&Objective: Heavy metals have a high resistance against degradation. So their amounts in human food chain, such as fish, may be increased even several times either in water or air, due to bioaccumulation. These amounts are potentially dangerous to human. This study was done to determin of heavy metals in edible tissue of Lutjans Coccineus and Tigeratooh Croaker in the Persian Gulf. Materials&Methods: 60 samples of Lutjans Coccineus and Tigeratooh Croaker were collected randomly after preparation and chemical digestion for trace elements determination. Pb, Cr, Cd and Ni were determined by flame atomic absorption in 2003. Results: Average value of Pb, Cr, Cd and Ni in the edible tissues of two fish species, Lutjans Coccineus 0.442,0.333,0.063 and 0.322 and Tigeratooh Croaker of were 0.48, 0.062, 0.064 and 0.48 ppm (dry weight basis), respectively. Conclusion: Results indicated that the mean concentrations due to heavy metals in these two species were less than acceptable limit of WHO, but related concentrations of Pb, Ni, Cd and Cr in 27%, 25%, 8% and 3% of individual studied samples respectively were more than upper acceptable limit of WHO.

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Background&Objective: Decrease in production of erythropoietin has been noted as one of the main factors causing anemia in ESRD patients, and administration of recombinant human erythropoietin (rhEPO) has been used to correct the anemia. Iron deficiency, including functional iron deficiency, limits the efficacy of rhEPO therapy in ESRD patients. This study examined the effects of maintenance intravenous iron sucrose (Venofer) on haemoglobin level and, optimization of erythropoietin therapy. Materials&Methods: Forty eight haemodialysis patients with haemoglobin level<9 gr/dl who were dialyzed three times weekly went under the study. Two thousands units of rhEPO were given subcutaneously at the end of each dialysis for seven weeks. At the end of the seventh week, those with haemoglobin level<9 gr/dl and with ferritin level <200 ng/dl (29 patients) were chosen for intravenous administration of 100 mg Venfor during the next five consecutive haemodialysis while maintaining the rhEPO dose at 2000 units with each dialysis. A week after the last dose of Venofer, haemoglobin and serum ferritin were determined. Results: Average haemoglobin level among the patients before administration of rhEPO was 7.5 gr/dl. After seven weeks of subcutaneous rhEPO at 2000 units with each haemodialysis, the average haemoglobin level raised to 8.5 gr/dl. The effect of maintenance IV Venofer was an increase in average haemoglobin level to 10.4 gr/dl. The same effect was seen on the ferritin level. The ferritin level of 131 ng/dl increased to 237 ng/dl a week after last dose of IV venofer. Conclusion: Intravenous (IV) iron improves haemoglobin response and, thus, optimizes rhEPO therapy.

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Background&Objective: Probiotics have been defined as live microorganisms that confer a health effect on the host when consumed in adequate amounts.Lactic bacteria play an important role in production and maintenance of the fermented and probiotic products.The aim of this study was to determine the efficacy of lactic bacteria, which isolated from yoghurt against Gastrointestinal pathogenic microorganisms in the Golestan province in North of Iran. Materials&Methods: In this descriptive study we used 96 strains which belong to 12 species of Lactobacillus and 5 species of Lactococcus ,that isolated from home made yoghurt, and their antimicrobial effect on 7 species of important intestinal pathogenic microorganisms were examined.After growing the Lactic bacteria in broth media centrifuged and filtred the suspension and used the supernatant for study.The effect of supernatant against intestinal pathogenic microorganisms were assessed by Disc diffusion and pour in well method and each test were repeated 3 times and mean inhibition zone were recorded. Results: Lactobacillus casei and Lactococcus lactis showed better effect than other strains.The maximum light zone diameter was 18 milimeter. Maximum and minimum inhibitiry effect has seen in Yersinia enterocolitica and Bacillus cereus. Conclusion: Both Lactobacillus and Lactococcus strains had a proper inhibitory effect on the intestinal pathogenic bacteria but Lactobacillus strains that present in yoghurt showed better effect. This inhibitory effect was more obvious on the Yersinia enterocolitica.

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Background&Objective: Considering the high prevalence of Tricomonas vaginalis (TV) in women and the known side effects of metronidazol, herbal drug therapy in order to reduce drug side effects has been considered increasingly in recent decades. This study was done to determine the effect of Artemisia aucheri Boiss, Zataria multiflora Boiss and Myrtus communis L. on Trichomonas vaginalis. Materials&Methods: This study was done on samples extracted from 100 patients with vaginitis due to Trichomona. The parasite was isolated from vagina and determined directly. Identification was done through direct smear preparation. Parasite was added to the 5 test tubes containing Dorse medium, metronidazole. dimethyl sulfoxaide (DMSO), Artemisia, Zataria and Myrtus extraction with concentration of 0.1, 0.01 ml in order to determine the effect of these concentrations within 72 hour. Results: Trichomonas could be alived in Dorse medium for 72 hours, in presence of metronidazole for one hour and in Dorse medium for 6 hours. Also, methonolic extracts of Artemisia are effective at concentration of 0.1 after one hour and 0,01 after 4 hours of the inoculation. Methanolic extracts of Zataria at concentrations of 0.1 and 0.01 and the Myrtus extract at concentration of 0.1 are effective at beginning of inoculation but the Myrtus extract at concentration of 0.01 is effective after one hour. Conclusion: Considering the acceptable effect of metanolic extracts of these plants on trichomonas in in-vitro conditions, it is recommended that , the therapeutic effects of the substances from these plants to be studied in in vivo conditions and in case of having positive effect to be used as a drug.

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Background&Objective: Hepatitis B, is a common kind of viral hepatitis. Vaccination is the most secure way of prevention for the health care worker. The present study was designed to survey the seropositivity of Gorgan teaching hospitals. Materials&Methods: This descriptive and cross-sectional study was done on 300 medical and official personals at 3 hospitals in Gorgan in 2003. with using 3ml of pripheral blood, sampling assay was performed to determine HBs-Ab and HBs Ag. Questionnaire form concerning demographic information, vaccination status and risk factors were compeleted for participants. Statistical analysis was done by means of SPSS soft ware with confidence interval equal to 95%. Results: 13% of individuals were not be vaccinated and 74.6% of them were vaccinated completely. There were statistically significant differences between HBs Ag and vaccination status ,individual’s age , the last vaccination time, occupation and use of glove (p<0.05). Furthermore, 1% of samples (one female, two males) were HBs Ag –positive. These three Persons has 10 years at service and were vaccinated compeletely were HBs –Ab neagtive. Conclusion: As a result of vaccination project, vaccination coverage of emplyees and immunologic status of them were satisfactory. Because of repetitive encountering of under considertion individuals with hepaptitis virus, 2% of unvaccinated subject had been infected but fortunately were be cured. Concomitant reduction in probability of immunity was being occurred after vaccination with the passing time and considering the importance of booster dose injection.

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Background&Objective: Genital mycoplasmas can cause infection of the genitourinary tract. Thses organisms are associated with bacterial vaginosis, pelvic inflammatory disease, endometritis, cervicitis, Nongonococcal urethritis. Spontaneous abortion, premature birth, neonatal pneumonia and meningitis, and infertility. The aim of this study was to determine the ability of PCR method for diagnosis and identification of genital mycoplasma in culture negative samples taken from women suffering from bacterial vaginosis. Materials&Methods: 174 genital samples were taken from women suffering from bacterial vaginosis during January until December 2005. Two genital swabs were taken from each patient. One of them was cultured on the mycoplasma specific media for isolation of mycoplasma. The other swab was immersed in PBS buffer and frozen until DNA extraction. To detect the presence of mycoplasma and ureaplasma in genital DNA Samples: a 520-bp fragment of the 16S rRNA was amplified. The specific primers used for this purpose were: MGSO, UGSO, MY- ins. Results: From 174 samples, 71 samples (40.8%) were positive by culture for mycoplasma & ureaplasma. From 103 culture negative samples. According to PCR results, 14 samples (13.6%) were positive and 89 Samples (86.4%) were negative for mycoplasma and ureaplasma. Conclusion: This study showed that PCR method is more sensitive than culture for detection genital mycoplasma, Therefore PCR is a rapid, sensitive and easy method to detect genital mycoplasmas in urogenital swabs.

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Background&Objective: Collection and Storage of roof rainwater usually has been used from long time ago in Iran and many other countries. Many residents in rural areas of Golestan province (Turkman Sahra) are providing partly of drinking and municipal water by this way. This study was purpose to determine the chemical water quality and detecting of probable contamination resources in cisterns. Materials&Methods: 140 samples were collected and analyzed for determination of chemical parameters during three seasons. Alkalinity by titrometric, hardness by E.D.T.A, Cl- by Mohr and No3- & PO4-3, by DR-2000 HACH and heavy metals by atomic absorption spectrometry (model 2380 Perkins-Elmer) were determined. These results were analyzed by SPSS and Kolmogorov-Smirnov test. Results: The chemical parameters such as: acidity, electrical conductivity, alkalinity, hardness, chloride, nitrate and manganese had desired limits but PO4- in 15%, Fe in 12%, Pb in 51% and Cr in 16% were exceeded the referrance level. Conclusion: Any of water cisterns were unfavorable for drinking because some chemical parameters and especially the heavy metals were exceeded the normal level. The probabe causes of the normal level chemical contamination water in cisterns include infiltration surface and agricultural waters, storage of Gorganrood water and precipitation of air pollution.

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Background & Objective: Pharmacological studies and traditional medical literature point to the cardiovascular effects of the Citrus aurantium L. (Rutaceae) in many instances. In the present study, we used isolated perfused AV-node of rabbit as an experimental model to determine the effect of various concentrations of essence of Citrus aurantium (0.1-0.3 v/v) on electrophysiological properties of isolated heart. The second goal of the present study was to assess the protective role of essence of Citrus aurantium on arrhythmogenic effects of ouabain. Materials & Methods: This was a semi-experimental study. We used isolated perfused rabbit AV-nodal preparation, in three groups. In the first group, the effect of various concentration of the Citrus aurantium (0.1, 0.2, 0.3 ml/l) was assesed on the AV-node. In the second and third groups, the effect of Citrus aurantium was tested in the presence of ouabain (0.1?molar) and verapamil (0.1?molar). We used 6 rabbit in each group. Statistical analysis was preformed by SPSS Mean±SE was used in the all results. Results: Our results showed depressant effects of extract of Citrus aurantium on Wenchebach Cycle Length (WBCL), AV Conduction Time (AVCT), Effective and Functional Refractory Periods (ERP & FRP). Rate-dependent properties such as Facilitation and fatigue significantly increased by Citrus.a (0.3ml/l). We had significant increase in the AVCT (32.6±3.6 to 40±6.08 msec) and FRP (147±5.1 to 166.6±3.6 msec) by Citrus.a. We had a protective role of Citrus aurantium on ouabain induced AV-nodal depression. The magnitude of facilitation and fatigue in the concentration of 0.3 C.aurantium was 7.5±0.3 and 6.5±0.5, respectively. Conclusion: The above results indicated potential inhibitory and antiarrhythmic effect of Citrus aurantium in treating supraventricular tachyarrhythmia.

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Background & Objective: Hypercholesterolemia is a risk factor for atherosclerosis that responded variably to dietary modification of fat and cholesterol. Previous studies in humans have shown that ingestion of lecithin can alter plasma cholesterol and triglyceride but results were different. The current study was designed to investigate the hypocholesterolemic properties of lecithin on patient with hypercholesterolemia. Materials & Methods: Sixty one subjects (27 Male, 34 Female) with mean ages 44±14 years old with hypercholesterolemia, was admitted to Mottahari Health Center(Shiraz), randomly assigned to the case (n=31) and control (n=30) groups. Case group received three lecithin capsules (1200 mg) which contain 420 mg Phosphatidylecoline, daily for six weeks. Subject's weight, Body Mass Index (BMI) and serum lipid concentration were measured at the beginning and the end of six weeks. Results: No significant difference were found in mean weight in case group when compared to control group. Also no significant difference were found in mean BMI in case group when compared to control group. Significantly lower mean serum Total Cholesterol, LDL cholesterol, and triglyceride and significantly higher HDL cholesterol were found in case group. In addition mean serum total cholesterol (P<0.05), LDL cholesterol (P<0.05) and TG (P<0.05) decreased and mean serum HDL-Cholesterol (P<0.05) increases significantly in case group vs. control group. Conclusion: This study showed that lecithin could be considered as effective nutrient useful in dietary treatment of hypercholesterolemia.

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Background & Objective: WHO estimated almost 27% of under 5 years children suffered from under-weight. This study aimed are assessing anthropometric indicators (underweight, wasting and stunting) in 0-24 months children at Golestan province and the relationship with the background variables of households. Materials & Methods: This cross-sectional study was done on 1473 children (under 2 years old) whom resided in urban and rural area. Samplimg method was single stage cluster sampling. Constitutional variables in child’s mothers, age, educational level, job and child’s weight and height are measured by structured questionnair. The data were analysed with chi square, chi square for trend and logistic regression. Results: The under-weight, wasting and stunting (mild to severe) prevalence were 21.4% (CI 95%: 19.3-23.6), 16.5% (CI 95%: 14.7-18.5) and 31.4% (CI 95%: 29.1-33.8) respectively. Maximum sex difference in the malnutrition indicators were seen stunting (boys 14.3%, girls 7.2%) (p<0.05). The prevalence of under-weight, wasting and stunting are positively correlated with increasing the children’s age. The under-weight and stunting linear trend was significant (p<0.05). The risk of under-weight children were 2.8 fold in illitraced mothers relative to mothers with high school diploma (CI 95% for adjusted odds ratio: 1.1-7). The risk was 1.5 fold in stunting (CI 95% for adjusted odds ratio: 0.5-4.3). Conclusion: The under-weight and wasting (moderate to severe) prevalence are increased relatively ANIS study (1998), but stunting prevalence are not increased. The mothers’ illitracy and very low educational level can be suspected risk factors for under-weight and stunting.

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Background & Objective: Mycobacterium marinum is the etiologic agent of fish Mycobacteriosis and fish tank granuloma in human in this study our objective was to determine of the fish tank granuloma in Fishermen and Mycobacterium marinum infection in Caviar fishes in Ashorade, in the Golestan province of Iran south east Caspian Sea border. Materials & Methods: Any suspected lesions in 387 subjects of Fishermen and 113 autopsy samples from gills of fish of Caviar were obtained and cultured in Lowenstein Jensen media. The mycobacterial species were determined by conventional biochemical tests. Results: No fish tank granuloma was proved in human, but 11 (9.73%) strains of Mycobacteria were isolated from Caviar fishes, that only 2 strains (1.76%) belong to M.marinum. Conclusion: This study showed that was low prevalence any cases of fish tank granuloma and infection due to M.marinum in Caviar fish in this Region.

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Background & Objective: Burnout is a syndrom that emerges by mental fatigue and loss of competency. Inappropriate pysical Environment and Professional Equipment may be caused Burnout in nursing. Therefore this study accomplished by object of determination of relationship between Burnout and physical Environment and professional equipments. Materials & Methods: This is a descriptive –analytical corrolational study. The environment of study was medical sciences hospitals and samples were nursing emploees in 1383-84. The research tools were two questionnaire including Maslach Burnout Inventory (MBI) consisted of three partsincluding: Emotional exhaustion, depersonalization and personal achivement. the next, was pysical Environment and Professional Equipments questionnaire. Results: Total participants were 272 nurses that43.8% of them had high level of emotional exhaustion, also there were a significant relationship between emotional exhaustion, dpersonalization and physical environment (p ? 0.05). In adition, there were a significant relationship between severity of emotional exhaustion and dpersonalization with professional equipments (p?0.05). There were a reversed relationship between burnout and professional equipments desirability. Conclusion: This study indicated that most of the samples had high emotional exhaustion and there were relationship with physical environment and professional equipments. Also there were a reversed relationship between burnout and professional equipments desirability.

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Background&Objective: To assess the effect of a lateral – wedge insole with subtalar supporting on the femorotibial angle in patients with varus defomrity of the knee. Materials&Methods: The efficacy of a wedged insole with subtalar supporting and that of traditional wedge insole shoe insert were compared. 20 outpatient with knee osteoarthritist were randomized to be treated with either the supported or traditional inserted insole. In both group , the baseline and 2 month koos scores for subjective knee pain and ADL and quality of live and symptoms were compared. Results: At the baseline , there were no significant difference in the femorotibial angle and pain and ADL, quality of life and symptoms, the 10 subjects wearing the subtalar supporting demonstrated a significatly increase femoretibial angle and koos scores without pain compared with insole group, there were no significant difference between twe group in pain after treatment. Conclusion: These results suggest that an insole with a subtalar supporting maintained the valgus correction of the, femoretibial angle in patgle in patients with varus knee OA for 2 months.

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Background & Objective: Diclofenac is a non-steroidal, anti-inflamatory drug that is prescribed as an analgesic. However, there is little known about the effects of diclofenac on the neural cells. In this study, we investigated the effects of diclofenac as sodium salt on the proliferation and differentiation of PC12 cells.

 

Materials & Methods: This expeimental study was done in Kerman neuroscience research center during 2004. The cell proliferation was evaluated by using XTT assay in the both free-serum neurobasal medium supplemented with B27 supplement and DMEM/F12 medium containing 10% FBS. The nerve growth factor(NGF) – induced differentiation was assessed  by measuring the neurite length for each treatment.

 

Results: The drug toxicity was exhibited at the higher concentrations of 310 mM in the supplemented neurobasal medium. The treatment of cells in the DMEM/F12 medium increased their sensitivity to diclofenac, with 40 and 85% growth inhibition at the 155 and 310 mM concentrations, respectively. The different generics of drug exhibited a equal toxic effects on the PC12 cells. The NGF- induced differentiation was not reduced by toxic and subtoxic concentrations of diclofenac.

 

Conclusion: This study indicated that diclofenac may be able to exhibit its neurotoxic effects through growth inhibition, but not differentiation inhibition. B27 supplement has several antioxidant compounds. Therefore, the difference of diclofenac cytotoxic effects in two culture media suggest that drug cytotoxicity may be related to the oxidative stress.

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Background & Objective: The effects of acute and chronic exposures to opiate drugs on anxiety process are controversial. Acute morphine injection showed the beneficial effects on anxiety. Morphine withdrawal induced severe anxiety response in morphine dependence rats. Whereas, the effects of chronic administrations of morphine on anxiety process are less studied. Furthermore, this study was designed to assess the role of morphine dependence on the level of anxiety in Rat. Materials & Methods: In this experimental study, Twenty male Wistar rats (250-300 gr) were made dependent by chronic administration of morphine in drinking water that lasted at least 21 days. Control groups received only sucrose in their water. This study utilized the elevated plus- maze model to evaluate anxiogenic-like behavior in rats. Four fundamental behavior patterns were recorded for 5 minutes: the time spent on open arms, the number of entries into open arms, stretched-attend posture and defecation. Immediately after test, the locomotor activity of each animal was tested by using an automated activity monitor system. The data were analyzed by independent t-test and two-way analysis of variance (ANOVA). Results: Finding indicated that the time spent on open arms and the numbers of entries into open arms were significantly shorter in morphine dependence group than control group (P<0.05). Also, the numbers of stretched-attend posture and defecation were significantly higher in morphine group (P<0.05). Whereas, there were no significant differences between groups in locomotor activity. Conclusion: This study showed that dependent rats may rapidly predispose anxiogenic- like effects in stressful conditions and without the effect on motor activity.