Journal of Gorgan University of Medical Sciences
مجله علمي دانشگاه علوم پزشكي گرگان
J Gorgan Univ Med Sci
Medical Sciences
http://goums.ac.ir/journal
59
journal58
1562-4765
2008-4080
10.61882/goums
fa
jalali
1388
12
1
gregorian
2010
3
1
12
1
online
1
fulltext
fa
ارتباط پلیمرفیسمهای موجود در ناحیه 874+ ژن IFN-Gama با عفونت نهفته هپاتیت B
The relation of polymorphisms in +874 region of IFN-Gama with occult HBV infection
ايمنيشناسي
Immunology
تحقيقي
Original Articles
<p style="font-style:normal;"></p>
<p dir="RTL" style="font-style:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">زمینه و هدف :</span></span></span> <span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">عفونت نهفته هپاتیت </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">B</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> شکل بالینی از بیماری هپاتیت </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">B</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> میباشد که در آن فرد علیرغم این که از نظر </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی است؛ اما دارای </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBV-DNA</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> در خون محیطی میباشد. هنوز علت عدم توانایی پاکسازی کامل ویروس از بدن مشخص نشده است. به نظر میرسد که تفاوتهای ژنتیکی و ایمونولوژیکی در این امر سهم عمدهای را دارا میباشند. از جمله مواردی که پاسخ ایمنی علیه ویروسها را دستخوش تغییر میکند؛ سیستم سیتوکینی است. این مطالعه به منظور تعیین ارتباط پلیمرفیسمهای موجود در ناحیه 874+ ژن </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">IFN-Gama</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> با عفونت نهفته هپاتیت </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">B</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> انجام شد.</span></span></span></span></p>
<p dir="RTL" style="font-style:normal;"></p>
<p dir="RTL" style="font-style:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">روش بررسی :</span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> در این مطالعه توصیفی ابتدا 3700 عدد پلاسمای تازه منجمد شده </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">(FFP)</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> که از نظر </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی بودند؛ جمعآوری و از نظر </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">anti-HBc</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> بررسی شدند. سپس نمونههای </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی و </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">anti-HBc</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت از نظر وجود </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBV-DNA</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> با روش </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">PCR</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> بررسی شدند. نمونههای </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBV-DNA</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت به عنوان موارد عفونت نهفته هپاتیت </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">B</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> از نظر پلیمرفیسمهای موجود در ناحیه 874+ ژن </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">IFN-Gama</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> با روش </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">ARMS-PCR</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مورد بررسی قرار گرفتند.</span></span></span></span></p>
<p dir="RTL" style="font-style:normal;"></p>
<p dir="RTL" style="font-style:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">یافتهها :</span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> نتایج این تحقیق روی نمونههای </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی نشان داد که 352 نمونه </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">(9.51%)</span></span></span></span> <span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">از این نمونهها از نظر </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">anti-HBc</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت بود. با انجام </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">PCR</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> روی نمونههای </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی و </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">anti-HBc</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت؛ مشخص گردید که 57 نمونه (%16.1 از افراد </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">anti-HBc</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت و </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBsAg</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> منفی) </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">HBV-DNA</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> مثبت هستند. دو گروه مورد و شاهد از نظر وجود تمامی ژنوتیپهای موجود در ناحیه 874+ ژن </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">IFN-Gama</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> تفاوتی نداشتند.</span></span></span></span></p>
<p dir="RTL" style="font-style:normal;"></p>
<p dir="RTL" style="font-style:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">نتیجهگیری : </span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;">این مطالعه نشان داد که هیچگونه ارتباطی بین پلیمرفیسمهای موجود در ناحیه 874+ ژن </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">IFN-Gama</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> و بیماری عفونت نهفته هپاتیت </span></span></span></span><span dir="LTR" style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:8.0pt;">B</span></span></span></span><span style="font-weight:normal;"><span style="color:navy;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:10.0pt;"> وجود ندارد.</span></span></span></span></p>
Background and Objective: Occult hepatitis B infection is a form of hepatitis in which despite of absence of detectable HBsAg, HBV-DNA is present in peripheral blood of patients. The mechanisms which are responsible for progression of OBI yet to be clarified but some investigators believed that the genetics and immunological parameters may are different in resistant individuals and patients. Cytokine network system could be leading alteration in viral immune response. The aim of this study was to investigate the relation between polymorphisms +874 region of IFN-Gama with occult hepatitis B infection. Materials and Methods: In this study, the plasma samples of 3700 blood donors were tested for HBsAg and anti-HBs by ELISA. The HBsAg negative and anti-HBc positive samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples assigned as occult hepatitis B infection cases and ARMS-PCR technique were performed to examine the present polymorphisms in +874 region of IFN-Gama genes of patients with occult hepatitis B infection. Results: 352 (9.51%) out of 3700 blood samples were negative for HBsAg and positive for anti-HBc antibody. HBV-DNA was detected in 57 (16.1%) of HBsAg negative and anti-HBc positive samples. Our results showed that there was not any significant difference between patients and control group in polymorphisms in +874 region of IFN-Gama genes. Conclusion: This study showed that there is not any significant difference between polymorphisms in +874 region with IFN-Gama occult hepatitis B infection.
عفونت نهفته هپاتیت B , IFN-Gama , پلیمرفیسم , HbsAg , HBV-DNA
Occult hepatitis B infection, IFN-Gama, Polymorphism, HBsAg, HBV-DNA
56
62
http://goums.ac.ir/journal/browse.php?a_code=A-10-1-433&slc_lang=fa&sid=1
Kazemi Arababadi M (PhD)
محمد
کاظمی عرب آبادی
5900319475328460051101
5900319475328460051101
Yes
Pourfathollah AA (PhD)
علی اکبر
پور فتح اله
5900319475328460051102
5900319475328460051102
No
Jafarzadeh A (PhD)
عبداله
جعفرزاده
5900319475328460051103
5900319475328460051103
No
Hassanshahi Gh (PhD)
غلامحسین
حسن شاهی
5900319475328460051104
5900319475328460051104
No
Daneshmandi S (MSc)
سعید
دانشمندی
5900319475328460051105
5900319475328460051105
No
Afrooz MR (BSc)
محمد رضا
افروز
5900319475328460051106
5900319475328460051106
No
Haddadian M (BSc)
محمود
حدادیان
5900319475328460051107
5900319475328460051107
No
Mohammadizadeh F (BSc)
فهیمه
محمدی زاده
5900319475328460051108
5900319475328460051108
No