fa اثر دیکلوفناک بر تکثیر و تمایز سلول‌های PC12 در محیط کشت عمومى General تحقيقي Original Articles <p dir="rtl" style="font-style:normal;"><span lang="FA" style="FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">زمینه و هدف :</span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"> دیکلوفناک از گروه داروهای ضدالتهابی غیراستروئیدی است که برای کاهش درد و التهاب تجویز می‌شود. اطلاعات اندکی در مورد اثر دیکلوفناک بر سلول‌های عصبی موجود است. این مطالعه به منظور بررسی اثرات دیکلوفناک سدیم بر تکثیر و تمایز سلول‌های </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">PC12</span><span dir="rtl"></span><span style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> <span lang="FA"><font size="2">در محیط کشت انجام شد.<p></p></font></span></span></p> <p dir="rtl" style="font-style:normal;"><span lang="FA" style="FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">روش بررسی:</span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"> این مطالعه تجربی در سال 1383 در مرکز تحقیقات علوم اعصاب کرمان انجام شد. تکثیر سلول در دو محیط کشت بدون سرم </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">Neurobasal</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> تکمیل شده با مکمل </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">B27</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> و محیط کشت </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">DMEM/F12</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> دارای 10درصد </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">FBS</span><span dir="rtl"></span><span style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> <span lang="FA"><font size="2">با روش </font></span></span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">XTT-assay</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> سنجش شد. تمایز سلولی القاء شده به وسیله عامل رشد عصب (</span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">NGF</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span>) با اندازه‌گیری طول نوریت‌های سلول بررسی شد.<p></p></span></p> <p dir="rtl" style="font-style:normal;"><span lang="FA" style="FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">یافته‌ها :</span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"> سمیت دارو در محیط کشت </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">Neurobasal</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> تکمیل شده با مکمل </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">B27</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> در رقت‌های بالاتر از 310 میکرومول مشاهده شد. سمیت در محیط کشت </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">DMEM/F12</span><span dir="rtl"></span><span style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> <span lang="FA"><font size="2">افزایش یافت، به ترتیبی که در رقت‌های 155 و 310 میکرومول رشد سلول 40 و 85 درصد کاهش یافت (05/0</font></span></span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">P<</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span>). ژنریک‌های مختلف دارو سمیت برابری بر سلول‌های </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">PC12</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> اعمال کردند. تمایز سلول </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">PC12</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> القاء شده با </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">NGF</span><span dir="rtl"></span><span style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> <span lang="FA"><font size="2">در رقت‌های توکسیک دارو نیز کاهش یا افزایش نیافت.<p></p></font></span></span></p> <p dir="rtl" style="font-style:normal;"><span lang="FA" style="FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">نتیجه‌گیری:</span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"> نتایج این مطالعه دلیل سمیت دیکلوفناک بر سلول‌های عصبی را از طریق تداخل در رشد سلولی و نه تمایز سلولی معرفی می‌کند. تفاوت سمیت سلولی دیکلوفناک در دو محیط کشت، با توجه به این که مکمل </span><span dir="ltr" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA">B27</span><span dir="rtl"></span><span lang="FA" style="FONT-WEIGHT: normal FONT-SIZE: 10pt FONT-FAMILY: Tahoma mso-bidi-language: FA"><span dir="rtl"></span> دارای چندین ترکیب آنتی‌اکسیدان است، استرس اکسیداتیو را به عنوان یکی از مکانیسم‌های سمیت دارو پیشنهاد می‌کند.<p></p></span></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"><font color="#000080"><font face="Times New Roman"><b><font size="3">Background & Objective: </font></b><span style="FONT-SIZE: 11pt">Diclofenac is a non-steroidal, anti-inflamatory drug that is prescribed as an analgesic. However, there is little known about the effects of diclofenac on the neural cells. In this study, we investigated the effects of diclofenac as sodium salt on the proliferation and differentiation of PC12 cells.<p></p></span></font></font></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"></p> <p><b><font color="#000080" face="Times New Roman" size="3">&nbsp;</font></b></p> <p></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"><font face="Times New Roman"><font size="3"><font color="#000080"><b>Materials & Methods:</b> </font></font><font color="#000080"><span style="FONT-SIZE: 11pt">This expeimental study was done in </span><city><place><span style="FONT-SIZE: 11pt">Kerman</span></place></city></font><span style="FONT-SIZE: 11pt"><font color="#000080"> neuroscience research center during 2004. The cell proliferation was evaluated by using XTT assay in the both free-serum neurobasal medium supplemented with B27 supplement and DMEM/F12 medium containing 10% FBS. The nerve growth factor(NGF) &ndash; induced differentiation was assessed<span style="mso-spacerun: yes"><font size="3">&nbsp; </font></span></font><font color="#000080">by measuring the neurite length for each treatment.<p></p></font></span></font></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"></p> <p><b><font color="#000080" face="Times New Roman" size="3">&nbsp;</font></b></p> <p></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"><font face="Times New Roman"><font size="3"><font color="#000080"><b>Results:</b> </font></font><span style="FONT-SIZE: 11pt"><font color="#000080">The drug toxicity was exhibited at the higher concentrations of 310 </font></span></font><font color="#000080"><span style="FONT-SIZE: 11pt FONT-FAMILY: Symbol mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-char-type: symbol mso-symbol-font-family: Symbol"><span style="mso-char-type: symbol mso-symbol-font-family: Symbol"><font face="Symbol" size="3">m</font></span></span><span style="FONT-SIZE: 11pt"><font face="Times New Roman">M in the supplemented neurobasal medium. The treatment of cells in the DMEM/F12 medium increased their sensitivity to diclofenac, with 40 and 85% growth inhibition at the 155 and 310 </font></span><span style="FONT-SIZE: 11pt FONT-FAMILY: Symbol mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-char-type: symbol mso-symbol-font-family: Symbol"><span style="mso-char-type: symbol mso-symbol-font-family: Symbol"><font face="Symbol" size="3">m</font></span></span><font face="Times New Roman"><span style="FONT-SIZE: 11pt">M concentrations, respectively. The different generics of drug exhibited a equal toxic effects on the PC12 cells.</span><span dir="rtl"></span></font><span dir="rtl" style="FONT-SIZE: 11pt FONT-FAMILY: Zar"><span dir="rtl"></span> </span><span style="FONT-SIZE: 11pt"><font face="Times New Roman">The NGF- induced differentiation was not reduced by toxic and subtoxic concentrations of diclofenac.<p></p></font></span></font></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"></p> <p><b><font color="#000080" face="Times New Roman" size="3">&nbsp;</font></b></p> <p></p> <p class="MsoNormal" style="TEXT-JUSTIFY: kashida MARGIN: 0cm 0cm 0pt DIRECTION: ltr unicode-bidi: embed TEXT-ALIGN: justify TEXT-KASHIDA: 0% mso-pagination: none"><font face="Times New Roman"><font size="3"><font color="#000080"><b>Conclusion:</b> </font></font><span style="FONT-SIZE: 11pt"><font color="#000080">This study indicated that diclofenac may be able to exhibit its neurotoxic effects through growth inhibition, but not differentiation inhibition. B27 supplement has several antioxidant compounds. Therefore, the difference of diclofenac cytotoxic effects in two culture media suggest that drug cytotoxicity may be related to the oxidative stress.<p></p></font></span></font></p> سمیت‌سلولی , دیکلوفناک , تمایز سلول عصبی , PC12 , عامل رشد عصب Diclofenac, Cytotoxicity, Differentiation, NGF, PC12 15 21 http://goums.ac.ir/journal/browse.php?a_code=A-10-1-254&slc_lang=fa&sid=1 Saeed Rajabalian سعید رجبعلیان 5900319475328460047965 5900319475328460047965 Yes Manzoomeh Shamsi Meimandi منظومه شمسی میمندی 5900319475328460047966 5900319475328460047966 No Shahryar Dabiri شهریار دبیری 5900319475328460047967 5900319475328460047967 No Rafat Hoseini رفعت حسینی 5900319475328460047968 5900319475328460047968 No